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doi: 10.1038/leu.2014.295 105 CuolloLDi CristofanoSSandomenicoAIaccarinoEOliverAZingoniAet al

Photobiol., B 209 , 111947 (2020)

It has been reported that MOTS-c improves age-related insulin resistance in male mice by increasing glucose intake in soleus muscles (20)

Cells were resuspended in a Low Salt Lysis Buffer (20 mM Tris-HCl, pH 8.0, 150 mM NaCl, 1 mM PMSF, and 3 mM 2-mercaptoethanol), lysed by sonication, and the soluble fraction isolated by centrifugation and incubated with low-density nickel agarose beads (GoldBio)