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Circadian disturbances are not likely to be an epiphenomenon of bipolar illness given that they are present during mania, depression, in euthymic state, and in healthy relatives of bipolar patients (181, 182)

S., Gatz, M., Gardner, C

2 h after adding the bacteria, infections were synchronized by washing cells with warm PBS to remove the extracellular bacteria, and IPTG was added at a final concentration of 0.05 mM to induce the expression of SdeA and its mutant