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The NEBNext Ultra Directional RNA Library Prep Kit for Illumina was utilised in conjunction with the NEBNext rRNA Depletion Kit (New England Biolabs) to perform ribo- and globin-depletion-based library preparation

Furthermore, evidence from multi-omics studies (Shakt et al., 2024), and the vascular depression hypothesis ( The interaction mechanism between AS and depression is highly complex, involving multiple potential mechanisms, including inflammation (Paranthaman et al., 2012), immune responses (Mousa et al., 2022), autonomic nervous system dysfunction (Pizzi et al., 2010), and intestinal flora and metabolite dysfunction (Wang A