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Cell culture and plasmid transfection HEK-293T cells were purchased from ATCC (Cat #CRL-3216)

Furthermore, uptake into cells was dependent on protein uptake rates, therefore increased uptake was achieved in BSA-medium (~ 26%) in contrast to only ~ 17% uptake in serum-medium (Barrouillet et al

Skin Aging and Collagen Loss Skin aging involves several structural changes: reduced collagen synthesis increased collagen degradation decreased elastin integrity slower cellular turnover These processes contribute to wrinkles, skin laxity, and reduced resilience