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Next, cDNA was synthesized by reverse transcription using the QuantiTect Reverse Transcription Kit from Qiagen according to the manufacturers instructions, and qRT-PCR was finally performed using a QuantiTect SYBR Green PCR Kit and QuantiTect Primer Assays (Qiagen) on a LightCycler 480 instrument (Roche)

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The diet enriched with a mixture of 0.5% propolis or 0.5% pollen is more effective than 1% propolis or 1% pollen alone on hematological parameters, plasma, and growth performance of rainbow trout (157)