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It is known that BAs may regulate multiple physiological and pathological processes, implicating a role in the progression of NAFLD/NASH ( Bacteroides and Clostridium , which produced DCA accumulation in the feces, serum, and liver, and hepatic DCA led to sucralose-induced NAFLD in mice ( Accumulating studies have demonstrated that amino acids might play a role in the process of NAFLD/NASH, but the relationship hasnt been elucidated

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Nevertheless, a significant dissimilarity of beta diversity on fecal microbiota was observed between healthy control and cirrhotic patients regardless of MDRO carriage ( p =0.001), as well as between cirrhotic patients with and without MDRO carriage ( p =0.033)

In fact, certain metabolites such as indole and salicylate could not be detected at all in a biological sample using IDA, whereas they could be quantified using MRM