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Patients exposed to harmful environmental chemicals such as toxins or pollutants can experience a speed-up in hormonal decline

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Immunoblotting Proteins were extracted from cultured SCD erythroblasts or spleen Ter119+ erythroblasts with modified RIPA buffer (150 mM NaCl, 50 mM Tris, pH 7.5, 1% NP40, 0.25% Na-deoxycholate) plus protease inhibitor cocktail (Sigma) and stored at 80 C up to processing

Slices of about 1.0 cm were collected from the two liver lobes and the median part