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To confirm GSTP1 knockdown in A2780DPP cells, we isolated individual puromycin-resistant colonies 24 h after colony formation using cloning cylinders, and extracted sufficient RNA to confirm GSTP1 knockdown by qRTPCR analysis (Figure 3B)

Whey protein is also used industrially in food products as it is a highly versatile ingredient

In order to test a possible effect of intracellular glutathione synthesis in such a process, buthionine sulfoximine (BSO), a specific the inhibitor of gamma-glutamylcysteine synthetase, was used to block the first step of intracellular glutathione synthesis

Clear regulatory guidelines and early engagement with agencies are also being pursued to ensure that promising ferroptosis-based CVD therapies can enter clinical trials and, ultimately, patient care