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Sahni JK, Doggui S, Ali J, Baboota S, Dao L, Ramassamy C (2011) Neurotherapeutic applications of nanoparticles in Alzheimers disease

doi: 10.1186/1471-2105-9-559

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The advantage of on-column tag removal is that it eliminates the extra step of separating the cleaved protein from GST, since the GST-tag itself remains bound to the medium