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The liver was further perfused with the perfusion buffer II (8.3 g/L NaCl, 0.5 g/L KCl, and 2.4 g/L HEPES), which was followed by collagenase type IV medium at 37 C

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Our analysis showed that CP-24 was absorbed into the bloodstream and could penetrate the blood-brain barrier following oral administration, though its concentrations were much lower than those of Osimertinib 34 (Supplementary Fig

The resulting acyl-phosphate intermediate undergoes nucleophilic attack by the -amino group of cysteine, which may be activated by an active site glutamate