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Recombinant proteins were analyzed using 10% SDS-PAGE and purified using HisTrap HP Crude Columns (GE Health) and AmiconUltra-15 Centrifugal Filter Units with Ultracel-3 membranes (Merck Millipore)

Long-term follow-up of dynamic brain changes in patients recovered from COVID-19 without neurological manifestations

The circular filter aliquots with a diameter of ten millimetres were cut from the Quartz filters and conducted the analysis