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High-throughput microscopy and quantitative analysis Images of wells from 96-well plates were acquired using the IN Cell Analyser 2500HS high-throughput microscope and plate reader (Cytiva)

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3.2 Internal tolerance mechanism 3.2.1 Cell wall modification Root cell wall is the principal barrier against harmful environmental cues and is considered a major target of Al (Yang and Horst, 2015

Simultaneously, anti-inflammatory cytokines such as TGF-, GDF-5, GDF-6, IL-4, and IL-10 are also present in the degenerative environment and can, to some extent, delay degeneration and alleviate pain (Wang et al., 2016), with their balance potentially closely related to the polarization state of macrophages (Yamamoto et al., 2022