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In mammalian cells the GPx4 protein is found in most subcellular compartments including the cytoplasm, the nucleus, mitochondria, and the endoplasmic reticulum (Arai et al., 1999)

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Once the samples were dried, transmission electron microscopy (JEM-1400Plus, Japan) was used to observe the ultrastructure of the tissue mitochondria to determine the occurrence and severity of ferroptosis

In the model with insomnia induced by PCPA shows the fissure in the tissues