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When either arm fails, its upstream metabolite accumulates: impaired MTR raises homocysteine, and impaired MUT raises methylmalonic acid (MMA)

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Analysis of HPLC Coupled with Tandem Mass Spectrometry (HPLCMS/MS) Optimization of the Mobile Phase The first mobile phases were methanol (solvent A) and water (solvent B) in a linear gradient from 0% to 15 % of methanol over 5 min, with the isocratic stage holding at 15 % methanol for 5 min

First, it inhibits the enzyme tyrosinase, which is the rate-limiting step in melanin production