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Cut the lotus leaf into a circle large enough to cover the bottom and sides of a medium steamer (such as a 10-inch/25 cm bamboo steamer), and place it there, pressing to make it fit snugly

Synbiotic formulations combining probiotics with prebiotics demonstrate synergistic effects, enhancing microbial diversity and metabolic functions more effectively than individual components (Raghani et al., 2024)

The author would like to thank Conselho Nacional de Desenvolvimento Cientfico e Tecnolgico (CNPq) for the productivity fellowship (CNPq 307022/2022-6)

Subsequently, the mixture was dried and washed with chloroform to remove uncapped free DOPC and then redispersed in chloroform solution that containing DOPC, cholesterol, PEG-2000 PE, and DOPE-RGD under sonicated for 15 min