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Gsk-3: tricks of the trade for a multi-tasking kinase

Following incubation, reactions were transferred to fresh tubes, and beads were washed four times with 125 l of 18 mM HEPES pH 7.4, 150 mM KOAc, 1 mM Mg(OAc) 2 , 5% (v/v) glycerol, 0.01% (v/v) IGEPAL CA-630 and 0.5 mM BME, with a tube change during the fourth wash

Varjosalo M, Keskitalo S, Van Drogen A, Nurkkala H, Vichalkovski A, Aebersold R, Gstaiger M (2013) The protein interaction landscape of the human CMGC kinase group

A double-blind RCT comparing patients treated with AZA and either prednisone 40mg daily tapered to 10mg daily or budesonide 3mg three times daily tapered to 3mg two times daily showed higher rates of serologic remission at six months (47% vs 18.4%, p 50% of patients