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Tracer injection and tissue harvesting To determine the specificity of connections made by PEG-fused sensory axons and their consequences for sensory terminal field organization, we used wheat germ agglutinin conjugated horseradish peroxidase (WGA-HRP) to anterogradely label sensory neurons

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Essential nutrients in the canine diet include: Macronutrients: amino acids (total protein) and essential fatty acids (fat) Amino acids: arginine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, threonine, tryptophan, and valine Essential fatty acids: omega 6 (linoleic acid) and omega-3 (alpha linolenic acid [ALA], eicosapentaenoic acid [EPA], and docosahexaenoic acid [DHA]) fats Macrominerals: calcium, phosphorous, sodium, potassium, chloride, and magnesium Trace elements: iodine, iron, manganese, selenium, zinc, and copper Vitamins: Vitamins A, D, E, and K