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The semi-quantitative PCR was set for the genes (NLRP3, caspase 1, ASC, gasdermin D, IL-1, Nf-Bp65, IL-6, GAPDH), which contained 100 l reaction volume consisting of 1x PCR buffer, a variable concentration of MgCl 2 , cDNA, forward and reverse primers, 200 M of dNTPs, and volume makeup with water

It resides in mitochondria, cytosol, and the nucleus of cells

As expected, BM ADO concentrations showed substantial inter-patient variability, consistent with previous reports describing wide fluctuations among MM patients ( Table 2 Individual patient values for ADO and INO concentrations in bone marrow and peripheral blood at baseline (pre-start) and at DARA treatment failure

Large-scale calcium waves traveling through astrocytic networks in vivo