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Forbes NS, Coffin RS, Deng L, Evgin L, Fiering S, Giacalone M, et al

To ensure robust and statistically reliable comparisons across treatments, we focused the downstream analysis on metabolites that passed quality filters and showed reproducible detection across all biological replicates in each treatment group (CK-stress, stress + UFe, stress + FFA, and CK non-stress), with all metabolite levels normalized to the CK non-stress baseline

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