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The cell viability was assessed by Hoechst staining of the nucleus (see Methods) and hence assessment of total cell numbers after 72 h of treatment, which is a good proxy for cell survival and cell growth upon prolonged compound exposure

However, Gill (142) found that short-term increases in systemic acetate and propionate levels did not alter Treg levels in mice, suggesting that the concentration of SCFA has a strong influence on the outcome of their effects in immunization

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