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By globally fitting the data to a 1:1 Langmuir binding model using Biacore Insight software (v6.0), the association and dissociation constants were determined

After washing 3 times for 10 mins in PBS, slices were incubated at RT on a shaker for 2 hours in secondary antibodies: goat anti-rabbit Alexa Fluor 594 (1:1000, Invitrogen, A11037) for MBP, goat anti-rabbit Alexa Fluor 488 (1:1000, Invitrogen, A11034) for Iba-1 and donkey anti-mouse Alexa Fluor 594 (1:1000, Thermo Fisher, A21203) for GFAP

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In conclusion, the photodegradation rates of profoxydim exhibited no significant difference between the paddy water and paddy soil matrices