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After anesthetizing C57BL/6 mice with 2% pentobarbital, the midline incision was taken to expose the bladder position of the mouse

Arthritis Rheum 1993

A key mechanistic insight is that IL-6 upregulates hepcidin, which promotes transferrin degradation and amplifies the vicious cycle of iron overload (Burton et al., 2020) (Figure 3D)