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During early follicular development, the follicular pool undergoes dynamic changes, marked by an increase in both primary and antral follicles

Similarly, drugs like atorvastatin have been repurposed to delay ARHL progression by activating pathways such as HSF1/Sirt1, which bolster antioxidant defenses and maintain mitochondrial structure (Choo et al., 2022)

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Definitive-screening design (DSD) is an alternative one-step design method that requires a smaller number of experimental tests to identify main effects by two-factor interactions but requires more suboptimal media designs to be tested