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, GRX1-roGFP2 or TRaQ-G) enable real-time, organelle-specific redox readouts but primarily report redox potential rather than absolute GSH levels, whereas fractionation coupled with mass spectrometry provides absolute GSH/GSSG quantification but remains static and susceptible to lysis-associated artifacts

After 12 h, the medium was replaced by normal growth medium containing 100 g/mL of cycloheximide (CHX, #C7698, Sigma-Aldrich, Shanghai, China)

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