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Inflammatory M1 phenotype macrophages exhibit increased oxidative stress, impaired cholesterol efflux and enhanced cytokine/chemokine secretion, leading to more LDL/remnant oxidation, endothelial cell activation, monocyte recruitment, and foam cell formation (54-59)

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Once the bacteria were normalized to OD 600 1 in LB chloramphenicol, an MOI of 100 was added to 0.5 10 6 primary murine macrophages from WT C57BL/6 or C57BL/6 deficient for phox ( gp91 phox-/- ) mice (The Jackson Laboratory, stock # 002365) that have been activated using 100ng/well recombinant murine IFN- (Peprotech, #315-05) for 18 hours

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