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Ratios of classical monocytes (CD14-/CD16-), which are more responsible for phagocytosis and initiation/priming of the immune system [254], are higher in patients with ALS and pre-symptomatic SOD1 carriers as compared with healthy controls, but function at a decreased efficacy and invade the CNS, though the difference between the roles of monocytes in the CNS versus systemic circulation are still unknown [255]

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The sequences of primers used for detecting the expression of target genes were as follows: -actin: F: GAAGGTGACAGCAGTCGGTT R: GGGACTTCCTGTAACAACGCA SOD2: F: CCCAGCAAGATAATGTCCTGTC R: AATGCAGACCTCTTTGATGGTT MMP1: F: AGGGGAGATCATCGGGACAA R: GCATCCCCTCCAATACCTGG PPAR: F: GAAAAGGCTATACGGACCACC R: TTTGTTGCCCTCAGCACAGT P21: F: TCTTGTACCCTTGTGCCTCG R: TGGTAGAAATCTGTCATGCTGGT Relative quantitative analysis of target gene mRNA expression was performed using the comparative Ct (Ct) method by normalizing the Ct values of the target genes to the Ct values of the internal reference gene -actin