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Description

The IEX purification protocol is initiated under low ionic strength, and the conditions are then changed so that the bound substances can be eluted differentially by increasing salt concentration or changing pH using a gradient or stepwise strategy

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Feeding mice a high-fat diet (HFD) can contribute to obesity, chronic low-grade inflammation, increased intestinal permeability, and ultimately worsen choroidal neovascularization by increasing the abundance of Firmicutes (Xiao et al., 2022)