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2.5 Cell viability assay HDFs were seeded at 8,000 cells/well in 96-well plates, treated for 24 h, and incubated with the CCK-8 reagent (APExBIO, K1018)

The mechanism during oxidative stress is different to the DDR response, in that ATM forms a disulphide linked stable dimer activated independently of MRN and DNA damage 50,51