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The sampling line length and fluid viscosity caused the pressure to drop before reaching the Duran bottle

Li H, Li L, Xue C, Huang R, Hu A, An X, et al

[34] Low-retention tips and calibrated micropipettes (or sterile transfer tools aligned with your SOP) [24] Alcohol prep (e.g., 70% IPA) for exterior wipe-down of vial stoppers and surfaces (per lab SOP) Labels for concentration, solvent, date/time, and storage condition Single-use aliquot tubes (recommended to avoid repeated freezethaw cycles) [34] Reconstitution Calculations: mg mL (Target Concentration) For research workflows, document concentration clearly (mg/mL or g/mL)

(PubMed) Labunskyy VM, Hatfield DL, Gladyshev VN