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100 l were used for perifusion for each group using a PERI4 machine (Biorep Technologies, Miami, FL, USA), allowing to run all three conditions in parallel

Afterward, samples were dehydrated through a graded alcohol series then paraffin-embedded, sectioned (5 m thickness), and stained with hematoxylin and eosin (H&E) to illustrate the general histological components with subsequent examination conducted under a light microscope (BX51, Olympus, Tokyo, Japan), and photomicrographs were acquired at 10X and 40X objectives (Paul and Chanda 2017)

Services were held at the Cromwell Funeral Home chapel in Hamburg with Rev