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To discover novel PPAR agonists, additional studies showed that 4-HDHA, 4-OXO-DHA and 17-OXO-DHA are strong PPAR transcriptional activators 21,22,23

doi: 10.3389/fnagi.2024.1444716 42

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Cryo-EM sample preparation and data acquisition CHIKV VLPs were incubated with a 2-f excess amount (molar ratio of Fab:VLP = 720:1) of C34 Fab or C37 Fab at 4 C in buffer containing 20 mM Tris, pH 7.5, 250 mM NaCl, 1 mM EDTA for 5 min, then prepared for VLP: Fab complex specimen preparation