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Description

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PEG-it Virus Precipitation Solution (LV810A-1, System Biosciences) was added to the clarified supernatant at a 1:4 ratio (e.g., 3.5 mL PEG-it per 14 mL supernatant), mixed, and incubated overnight at 4 C on a rotator

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The Gateway destination vector pAG415GAL-ccdB (CEN/ARS-based, GAL1 promoter, and LEU2 marker) was purchased from Addgene and served as the yeast expression vector