glutathione sepharose affinity column Sepharose™ 4 Fast Flow Cytiva 17-5132-01, pack of 25 mL Applications Affinity purification of proteins with a GST tag. gst glutathione affinity How to

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At each sampling point, cellular Fe 2+ was oxidised to Fe 3+ by addition of potassium dichromate (40 mM final concentration) to prevent further peroxide bond activation, followed by protein precipitation with acetonitrile (containing 150 ng/mL of diazepam as the internal standard) as previously described 28