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In the blank cuvette, distilled water (10 l) was added to monobromobimane (stock solution 30 l) in presence of phosphate buffer (3.0 ml)

Annu Rev Pharmacol Toxicol 53:401426

10.4155/tde.12.6 41 MagnaniM.RossiL.BiagiottiS.BianchiM

Accordingly, despite losing 25% of their body mass, GLP-1MK-801-treated mice maintained an energy expenditure that was similar to that of vehicle-treated control mice and was therefore also significantly higher than the gradually decreasing energy expenditure observed in mice that were calorie-restricted to match the weight loss trajectory of GLP-1MK-801-treated mice