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In our previous study using MB49 tumor, we also demonstrated that inflammatory macrophages (M1) is an important cell subpopulation present in TME of BCG treated animals ( + T cells in subcutaneous MB49 bladder tumors ( In summary, our data brings insight in the importance of BCG immunotherapy against melanoma, demonstrating that the intratumoral treatment with BCG in C57BL/6 mice induces the recruitment of a robust inflammatory cells infiltrate (M1-like tumor-associated macrophages, neutrophils, dendritic cells, CD4 + and CD8 + T cells, NK and NKT cells) and polarize the TME to a hot profile (antitumoral) associated with tumor control