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In complex biological mixtures such as cell lysates, the liquid chromatography-based resolution is not optimized for any one metabolite, and therefore some metabolites of interest (e.g., cystathionine) may not be readily identified

A number of oncogenic and tumor suppressor factors are also involved in cholesterol biosynthesis, for example, p53 plays a huge role in the mevalonate pathway (Loughran and Emerling, 2022)