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Another study demonstrated beneficial effects of Yuzu extract via inhibition of cox-2 and suppression of monocyte chemotactic protein, TNF, interleukins, p38 phosphorylation and nitric oxide synthase in RAW264.7 cells

3-hydroxy-N-trimethyllysine is then cleaved into gamma-trimethylaminobutyraldehyde, a reaction catalyzed by hydroxyl N-trimethyllysine aldolase (HTMLA)[25]

Fractions were analysed by western blotting, where the signal intensities of each fraction were quantitated and analysed using the Multi Gauge software (version 3.0, Fujifilm)

Methodological caveat: Most human trials use combination formulations published studies often combine GHK-Cu with other actives (e.g., retinoids, vitamin C), making it difficult to isolate GHK-Cus independent contribution