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Sustained IGF1R activation across that timeline using native IGF-1 would require multiple daily media changes

doi: 10.1111/j.1365-2362.2011.02567.x 403 ChaudhuriABehanPO

Erythrocyte free glycine and erythrocyte glutathione-derived glycine were converted to the n -propyl ester heptafluorobutyramide derivatives, and the isotope ratio of each was measured by negative chemical ionization gas chromatographymass spectrometry on an Agilent 6980 gas chromatograph complexed to a 5973 mass spectrometer (Agilent Technologies), monitoring ions at an m/z of 293 to 295

Succinate links TCA cycle dysfunction to oncogenesis by inhibiting HIF- prolyl hydroxylase