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User experimentation suggests that longer initial treatment periods (6+ months continuous) support better benefit retention during subsequent breaks, complete discontinuation breaks of 1-2 months show acceptable benefit maintenance for most users, and very extended breaks (3-6+ months) increase risk of regression toward pre-treatment baseline particularly for short initial treatment courses

10.1007/s00005-009-0019-8 105 NavakkodeS.SajikumarS.FreyJ

In the presence of legumain, AuNP-A&C-R exhibited aggregation properties due to the cleavage-generated thiolamino group, allowing click cycloaddition with cyanobenzothiazole under physiological conditions, as previously reported by Rao et al

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