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For reference range verification, the samples were frozen within 30-60 minutes from collection

Immunogenic cell death of breast cancer stem cells induced by an endoplasmic reticulum-targeting copper(II) complex

Before a typical experiment, 135,000140,000 cells were seeded in a 6-cm dish and cultured at a density of 37,000 cells per 12-well plate and grown for 4446 h before transfection