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By integrating research findings from PCC and ME/CFS, we seek to construct a pathophysiological framework that may account for multi-system interactions, potentially providing a more comprehensive understanding of the onset and progression of PEM

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After the membrane was blocked with 5% skim milk TBST (20 mM Tris-Cl (pH 7.4), 150 mM NaCl, 0.1% Tween-20), it was incubated with streptavidin (SA) coupled to horseradish peroxidase (HRP) (1:1000, Pierce) and washed three times with TBST for 10 min

Glutathione peroxidases 4 (GPX4) is a selenocysteine-containing, and GSH-dependent monomeric enzyme (Kelner and Montoya, 1998