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140 In contrast, growth of the CT-2A Astrocytoma in these mice was not influenced by an unrestricted KD containing 17E% protein, 141 but significantly reduced on a calorically restricted KD containing only 8E% protein, along with significant reductions in blood glucose and IGF-1

The resulting bone marrow cells were then adjusted to a final concentration of 3.510 6 cells/ml in DMEM + media, and 100 l were added per well of a 96-well plate for the survival assays

As the rate-limiting enzyme of the SSP, PHGDH high expression is closely correlated with the malignancy of PDAC, and targeting PHGDH effectively inhibits the growth of pancreatic cancer cells under nutrient-deprived conditions (74, 76)