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Documentation of the research purpose, careful sourcing, and meticulous record-keeping reflect this research orientation

Cell Lysates: For adherent cells, gently wash the cells with a moderate amount of pre-cooled PBS and dissociate them using trypsin

Approximately 0.3% (1:300) of patients of European or African ancestry have two loss-of-function alleles of the TPMT gene and have little or no TPMT activity (homozygous deficient or poor metabolizers), and approximately 10% of patients have one loss-of-function TPMT allele leading to intermediate TPMT activity (heterozygous deficient or intermediate metabolizers)

Pomegranate-derived compounds have been shown to modulate a wide range of molecular targets, including transcription factors, pro- and anti-apoptotic proteins, cell cycle regulators, protein kinases, cell adhesion molecules, pro-inflammatory mediators, and growth factors across multiple cancer types, such as skin, breast, prostate, colon, and lung cancers (366, 367)