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An Afe I restriction site directly upstream of the Strep -tag II, together with the unique Xba I restriction site in front of the whole reading frame, was subsequently used to exchange the entire coding region for sfGFP by the ones for different other POIs (see Supplementary Table S1)

Protein methylation was measured by standard immunoblotting using the following antibodies and dilutions: Abcam ab23366, 1:500, for mono- and di- methylated lysine, Abcam ab5823, 1:500, for histone 4 Arginine 3 symmetric demethylation, Abcam ab8580, 1:1000, for Histone H3 Lysine 4 trimethylation, Sigma A5441, 1:10000, for actin, and Abcam ab412 clone 7E6, 1:500, for monomethylated/dimethylated arginine

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It may not interfere with fasting goals for fat loss or insulin control