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Other studies have found cerebrospinal fluid levels of iron and ferritin (stored iron) were lower and levels of transferrin (an iron transport protein) were higher in patients with RLS compared with people without the condition (Earley 2000

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1 H-NMR(D 2 O, ppm): 2.32.5(m, 2H, CH 2 CO 2 Na), 2.62.9(m, 2H), 3.23.3(m, 1H, 3-H) 13 C-NMR(D 2 O, ppm): 40.89(CH 2 CO 2 Na), 48.25(4-CH 2 ), 51.10(3-CH), 179.37(CO 2 Na) Calcium hydroxide (340 mg, 4.59 mmol) was added to the reacting solution of D 2 O containing (S)-4-hydroxy-3-methanesulfonyl hydroxybutyric acid, so prepared from the Example 3 and stirred at room temperature for 30 minutes