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Low serum B12 can mean that tissues are competing for limited holoTC in the bloodstream, and areas with high B12 turnover (including the bone marrow) may get priority, while the brain, liver and peripheral nerves do not receive enough

According to predictions from the KEGG database, metformin has been shown to significantly increase the activity of glutamate-cysteine ligases in microbial metabolism

For example, depending on the assay, serum B12 cutoffs for deficiency for adults may range from 120 to 180pmol/L, with 148pmol/L based on 3SDs from the mean of an adult reference range, said to be the most common cutoff for frank deficiency (IOM, 2000

This revealed that BSO treatment drastically reduced endogenous glutathiones in RGC-5 cells, a result that is consistent with previous findings 15 , and also led to a higher susceptibility of the cells to H 2 O 2 -mediated oxidative stress (Supplementary Figure S4)