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The remaining buffer was carefully removed and 3flag-tagged proteins were eluted using 90 l of flag elution buffer (20 mM HEPES-KOH (pH 7.9), 150 mM KCl, 1 mM DTT and 1 mg/ml 3flag peptide, Sigma-Aldrich, F4799)

H.SreevidyaC.LachmansinghJ.MorrisJ.et al

Gene Therapies Targeting Mitochondrial Dysfunction in Parkinson's Disease Gene therapy entails the treatment of disease via delivery of a transgene that either replaces or corrects a defective gene or is generally supportive of cells in the disease environment (O'Connor and Boulis, 2015)