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After they were used for (H 2 O) n -GCIB-SIMS imaging, frozen skin sections on gold-coated silicon wafers were kept first at 20C for 1 hour, then at 4C for 1 hour, followed by fixation in 4% formalin solution at 4C for 10 minutes and methanol at 20C for 10 minutes

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Whether declining MOTS-c is a cause or consequence of this deterioration is still being resolved, but the correlation is striking enough that some researchers have proposed plasma MOTS-c as a biomarker of mitochondrial vitality and metabolic age

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doi: 10.1016/j.jep.2020.112792 67 LuoD.LiuY.WangY.ZhangX.HuangL.DuanB

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