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It also exerts neuroprotective effects by directly or indirectly inhibiting the gene expression of glycogen synthase kinase 3 (GSK3) [56, 57]

Shen Y, Hong J, Asaka R, Asaka S, Hsu F, Suryo Rahmanto Y, et al

For Co-IP, cell lysate was prepared for WB assay, and 1.01.2 mg proteins were incubated with normal rabbit IgG (as control) for 2 h, and followed by 2 h incubation with 20 L protein A/G-beads