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The dissected brains were incubated in 100 nM TMRE solution (Thermo Fisher Scientific) in DSM for 10 min and relative fluorescence intensity was quantified by top excite-read method through Synergy H1 reader

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It is worth noting that the changes in SIRTs activity and expression are closely related to the pathophysiological processes of kidney disease, including AKI and CKD (Perico et al., 2024)

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